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刘俊杰 博士

助理教授


2007-2011,四川大学--生命科学与技术基地班,学士

2011-2016,北大-清华-NIBS联合培养(PTN)项目,博士

2016-2018,劳伦斯-伯克利国家实验室--生物物理和生物成像中心,博士后

2018-2020,加州大学伯克利分校--细胞分子生物学系,博士后

2018-2020,美国生命科学研究基金(LSRF)研究员

2020,10月起,清华大学--生命科学学院、生命科学联合中心、高精尖结构生物学中心,生物结构前沿中心,助理教授

 

●  研究兴趣、领域:


本实验室主要利用生物信息学,生物化学,生物物理学以及细胞生物学手段从事以下研究

(1) 新型基因编辑工具的设计和开发

(2) 与RNA相关联的核酸酶机器的工作机理研究

(3)重大疾病相关的lncRNA结构功能研究及潜在治疗手段开发


●  代表性论文:


1. Tsuchida CA, Zhang SY, Doost MS, Zhao YQ, Wang J, O’Brien E, Fang H, Cheng-Ping Li, Li DY, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA, Liu JJG*. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Molecular Cell (2022).

2.Yang MY, Sun RR, Deng PJ, Yang YZ, Wang WJ, Liu JJG*, Chen CL*. Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem. Sci (2021). (Co-responding Author)

3. Liu TY#, Liu JJ#, Aditham AJ, Nogales E, Doudna JA. Target preference of Type III A CRISPR-Cas complexes at the transcription bubble. Nature Communications,10(1), 3001(2019). (Co-first author)

4.  Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knot GJ, Harrington L, Al-Shayeb B, Wanger A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA. CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature, 566,218-223 (2019).

5. Jiang F#, Liu JJ#, Osuna BA#, Xu M, Berry JD, Rauch BJ, Nogales E, Denomy JB, Doudna JA. Temperature-responsive competitive inhibition of CRISPR-Cas9. Molecular Cell, 1016-016(2018). (Co-first author)

6. Wright AV#, Liu JJ#, Knott GJ, Doxzen KW, Nogales E, Doudna JA. Structures of the CRISPR genome integration complex. Science, 357, 1113-1118 (2017). (Co-first author)

7. Shing J#, Jiang F#, Liu JJ#, Bray NL, Rauch BJ, Baik SH, Nogales E, Bondy-Denomy J, Corn JE, Doudna JA. Disabling Cas9 by an anti-CRISPR DNA mimic. Science Advances, 3(7): e1701620(2017). (Co-first author)

8. Ma M#, Liu JJ#, Li Y, Huang Y, Ta N, Chen Y, Fu H, Ye MD, Ding Y, Huang W, Wang J, Dong MQ, Yu L, Wang HW. Cryo-EM structure and biochemical analysis reveal the basis of the functional difference between human PI3KC3-C1 and-C2. Cell Research, 27,989-1001(2017). (Co-first author)

9. Liu JJ, Niu CY, Wu Y, Tan D, Wang Y, Ye MD, Dong MQ, Huang N & Wang HW, CryoEM structure of yeast cytoplasmic exosome complex, Cell Research, 26, 822-837(2016).

10. Liu JJ, Bratkowski MA, Liu XQ, Niu CY, Ke AL & Wang HW, Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM, Nature Structural & Molecular Biology, 21, 95–102 (2014).

 

●  联系方式:


电子邮件:junjiegogoliu(at)tsinghua.edu.cn (联系时请把(at)改成@)

实验室网站:http://gogolab.life.tsinghua.edu.cn

电话:+86-010-62770270 (lab)    +86-010-62770276 (office)

实验室通讯地址:北京市海淀区清华大学生物医学馆A401