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陈春来 博士

副教授


1999-2003      北京大学化学与分子工程学院        学士

2003-2008     北京大学化学与分子工程学院         博士

2008-2015      University of Pennsylvania             博士后

2015-2018      银河国际app手机版下载 - 最新版APP Store                   助理教授

2018-至今       银河国际app手机版下载 - 最新版APP Store                   副教授

 

研究兴趣、领域


1) 发展新型单分子荧光技术;

2) 蛋白在DNA上的靶向搜寻机制的研究;

3) CRISPR系列蛋白靶向和剪切机制的研究。

 

代表性论文


1.  Jingfei Xu(#), Lingyun Zhao(#), Sijia Peng(#), Huiying Chu(#), Rui Liang, Meng Tian, Philip P. Connell, Guohui Li (*), Chunlai Chen (*) and Hong-Wei Wang (*), (2021) Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination, Nucleic Acids Research, 10.1093/nar/gkab1141

2.  Xiaolin Niu(#), Ruirui Sun(#), Zhifeng Chen, Yirong Yao, Xiaobing Zuo, Chunlai Chen (*) and Xianyang Fang (*), (2021) Pseudoknot length modulates the folding, conformational dynamics and robustness of Xrn1 resistance of flaviviral xrRNAs, Nat Commun, 10.1038/s41467-021-26616-x

3.  Mengyi Yang, Ruirui Sun, Pujuan Deng, Yuzhuo Yang, Wenjuan Wang, Jun-Jie Gogo Liu (*) and Chunlai Chen (*), (2021) Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search, Chem Sci, 12, 12776-12784

4.  Ming-Jie Han(#), Qing-tao He(#), Mengyi Yang(#), Chao Chen, Yirong Yao, Xiaohong Liu, Yuchuan Wang, Zhong-liang Zhu, Kong-kai Zhu, Changxiu Qu, Fan Yang, Cheng Hu, Xuzhen Guo, Dawei Zhang, Chunlai Chen (*), Jin-peng Sun (*) and Jiangyun Wang (*), (2021) Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction, Chem Sci, 12, 9114-9123

5.  Sijia Peng (#), Weiping Li (#), Yirong Yao (#), Wenjing Xing, Pilong Li (*) and Chunlai Chen (*), (2020) Phase separation at the nanoscale quantified by dcFCCS, PNAS, 117(44):27124-27131

6.  Sijia Peng (#), Xiaowei Wang (#), Lu Zhang (#)(*), Shanshan He, Xin Sheng Zhao (*), Xuhui Huang (*) and Chunlai Chen (*), (2020) Target search and recognition mechanisms of glycosylase AlkD revealed by scanning FRET-FCS and Markov State Models, PNAS, 117(36):21889-21895

7.  Man Pan (#), Qingyun Zheng (#), Shan Ding (#), Lujia Zhang (#), Qian Qu, Tian Wang, Danning Hong, Yunjing Ren, Lujun Liang, Chunlai Chen (*), Ziqing Mei (*), and Lei Liu (*), (2019) Chemical Protein Synthesis-Enabled Mechanistic Studies on the Molecular Recognition of K27-linked Ubiquitin Chains, Angew. Chem. Int. Ed, 58(9):2627-2631

8.  Bo Wu, Haibo Zhang, Ruirui Sun, Sijia Peng, Barry S Cooperman, Yale E Goldman (*), and Chunlai Chen (*), (2018) Translocation kinetics and structural dynamics of ribosomes are modulated by the conformational plasticity of downstream pseudoknots, Nucleic Acids Research, 46(18): 9736-9748

9.  Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports, 22(2): 372-382

10.  Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2018) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, 9(10): 890-895

11.  Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885

 

联系方式


邮箱:chunlai@tsinghua.edu.cn